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soluble anti cd28 antibodies  (Bio X Cell)


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    Structured Review

    Bio X Cell soluble anti cd28 antibodies
    Soluble Anti Cd28 Antibodies, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/soluble+anti+human+cd28/InVivoMAb+anti-human+CD28/pm41865369-356-37-43
    Average 95 stars, based on 76 article reviews
    soluble anti cd28 antibodies - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Purification:

    Article Title: Potent antitumor activity of a designed interleukin-21 mimic
    Article Snippet: CD4 + and CD8 + T cells were then purified by negative selection and magnetic separation (Stem Cell Technologies Cat# 17952 and Cat# 17953). .. Purified human CD4 + and CD8 + T cells were activated by plate-bound anti-human CD3 (2 μg/mL; clone UCHT1 (Leu-4) (T3), BioXCell Cat# BE0231) and soluble anti-human CD28 (1 μg/mL; clone 9.3, BioXCell Cat# BE0248) and cultured in complete RPMI medium for 48 hours at 37°C. .. Murine B cells were isolated from the spleens of 6- to 8-week-old C57BL/6 mice through negative selection and magnetic separation (Stem Cell Technologies Cat# 19854).

    Article Title: Design of a potent interleukin-21 mimic for cancer immunotherapy
    Article Snippet: .. Purified human CD4 + and CD8 + T cells were activated by plate-bound anti-human CD3 (2 μg/mL; clone UCHT1 (Leu-4) (T3), BioXCell Cat# BE0231) and soluble anti-human CD28 (1 μg/mL; clone 9.3, BioXCell Cat# BE0248) and cultured in complete RPMI medium for 48 hours at 37°C. ..

    Cell Culture:

    Article Title: Potent antitumor activity of a designed interleukin-21 mimic
    Article Snippet: CD4 + and CD8 + T cells were then purified by negative selection and magnetic separation (Stem Cell Technologies Cat# 17952 and Cat# 17953). .. Purified human CD4 + and CD8 + T cells were activated by plate-bound anti-human CD3 (2 μg/mL; clone UCHT1 (Leu-4) (T3), BioXCell Cat# BE0231) and soluble anti-human CD28 (1 μg/mL; clone 9.3, BioXCell Cat# BE0248) and cultured in complete RPMI medium for 48 hours at 37°C. .. Murine B cells were isolated from the spleens of 6- to 8-week-old C57BL/6 mice through negative selection and magnetic separation (Stem Cell Technologies Cat# 19854).

    Article Title: Design of a potent interleukin-21 mimic for cancer immunotherapy.
    Article Snippet: JungHo Chun†, Birkley S. Lim†, Suyasha Roy†, Michael J. Walsh†, Gita C. Abhiraman†, Kevin Zhangxu, Tavus Atajanova, OrYam Revach, Elisa C. Clark, Peng Li, Claire A. Palin, Asheema Khanna, Samantha Tower, Rakeeb Kureshi, Megan T. Hoffman, Tatyana Sharova, Aleigha Lawless, Sonia Cohen, Genevieve M. Boland, Tina Nguyen, Frank Peprah, Julissa G. Tello, Samantha Y. Liu, Chan Johng Kim, Hojeong Shin, Alfredo QuijanoRubio‡, Kevin M. Jude, Stacey Gerben, Analisa Murray, Piper Heine, Michelle DeWitt, Umut Y. Ulge, Lauren Carter, Neil P. King, DanielAdriano Silva‡, Hao Yuan Kueh, Vandana Kalia, Surojit Sarkar, Russell W. Jenkins, K. Christopher Garcia, Warren J. Leonard*, Michael Dougan*, Stephanie K. Dougan*, David Baker*

    Article Title: Design of a potent interleukin-21 mimic for cancer immunotherapy
    Article Snippet: .. Purified human CD4 + and CD8 + T cells were activated by plate-bound anti-human CD3 (2 μg/mL; clone UCHT1 (Leu-4) (T3), BioXCell Cat# BE0231) and soluble anti-human CD28 (1 μg/mL; clone 9.3, BioXCell Cat# BE0248) and cultured in complete RPMI medium for 48 hours at 37°C. ..

    Article Title: BLIMP1 negatively regulates IL-2 signaling in T cells
    Article Snippet: CD4 + T cells were purified from PBMCs by negative selection following the kit’s protocol (#17952, Stem Cell Technologies). .. CD4 + T cells were then activated with plate-bound anti-human CD3 (2 μg/ml, #BE0001-2; clone OKT3, BioXCell) and soluble anti-human CD28 (1 μg/ml; #BE0248; clone 9.3, BioXCell) and cultured in RPMI 1640 medium with 10% FBS, 2 mM l -glutamine, and penicillin (50 U/ml) + streptomycin (50 μg/ml) in the presence of recombinant human IL-2 (100 IU/ml) for 72 hours at 37°C. ..

    Recombinant:

    Article Title: Polyfunctional IL-21 + IFNγ + T follicular helper cells contribute to checkpoint inhibitor diabetes mellitus and can be targeted by JAK inhibitor therapy
    Article Snippet: Peripheral blood mononuclear cells were isolated from whole blood by density gradient centrifugation using Ficoll-Paque (Cytiva). .. For Tfh skew, cells were stimulated with plate bound anti-human CD3 (BioXcell, clone UCHT1; 1 ug/mL) and soluble anti-human CD28 (BioXcell, clone 9.3; 1 ug/mL), recombinant human IL-12 (PeproTech; 10 ng/mL) and Activin A (R&D Systems; 100 ng/mL), as previously described . ..

    Article Title: BLIMP1 negatively regulates IL-2 signaling in T cells
    Article Snippet: CD4 + T cells were purified from PBMCs by negative selection following the kit’s protocol (#17952, Stem Cell Technologies). .. CD4 + T cells were then activated with plate-bound anti-human CD3 (2 μg/ml, #BE0001-2; clone OKT3, BioXCell) and soluble anti-human CD28 (1 μg/ml; #BE0248; clone 9.3, BioXCell) and cultured in RPMI 1640 medium with 10% FBS, 2 mM l -glutamine, and penicillin (50 U/ml) + streptomycin (50 μg/ml) in the presence of recombinant human IL-2 (100 IU/ml) for 72 hours at 37°C. ..



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